JAPANEASEENGLISH
HOMECompanyProductsServicesContactLinksSitemapPDF Brochure

Plant Science Instruments / FluorCams

 

Kinetic Imaging Fluorometer FluorCam

FluorCams are imaging fluorometers that measure sequences of Chl-fluorescence images with user-defined irradiance protocols and timing of measurements. Fluorescence emission is induced by two (or more) panels of super-bright, light emitting diodes (LEDs) that provide measuring flashes. Photochemistry is driven by continuous actinic irradiance and/or by strong saturating pulses of light that are generated in a halogen lamp, equipped with a shutter, or in supplemental LED panels. Fluorescence images are captured by a CCD camera. The images are taken at 12-bit resolution in 512 x 512 pixels of a CCD chip. Up to fifty images can be taken in one second. Data are transmitted via a USB 2.0 port to a desktop computer or to a notebook. FluorCams are supplied with a software package that includes a Wizard with the most frequently used experimental protocols. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.

 

 

Closed FluorCam 701MF Closed Version

Closed FluorCam consists, in its core, of a CCD camera, two panels of light emitting diodes (LEDs), and of a halogen lamp with a shutter. All these components are arranged around a sample chamber. The CCD camera equipped with a F1.2/2.8-6 mm objective captures 512 x 512 pixel images of 12-bit (or 14-bit) resolution with maximal frequency of 50 frames/sec. Images are recorded synchronously with measuring light flashes. The data are communicated via USB 2.0 interface.

Closed FluorCam 701MF@Closed Version

 

Open FluorCam 701MF Open

Open FluorCam is a highly modular instrument with flexible geometry. The LED panels and the light source generating saturating flashes can be arranged at various angles and distances from the sample. Also, the position of the camera relative to the sample is adjustable. The instrument can be configured with various types of LED panels and supplemental light sources. The other essential features of theinstrument are similar to the FluorCam closed version.

Open FluorCam 701MF Open

 

Micro FluorCam 701MF Micro-Scope Version

Micro-FluorCam (Kuepper et al. 2000, Photosyntetica 38 (4): 553-570, Ferimazova et al. 2002, Photochem. Photobiol. 76, 501-508) extends the complete capacity of kinetic Chl-fluorescence imaging to the realm of individual cells and sub-cellular structures. All conventional fluorescence parameters can be mapped with micrometerresolution so that individual chloroplasts or even grana-stroma thylakoid segments can be investigated (Vacha et al. 2005, Micron 36 (6): 483-502). Micro-FluorCam can be mounted to a wide range of microscopes.

Micro FluorCam 701MF@Micro-Scope Version

 

Handy FluorCam 701MF Handy Version

Handy FluorCam is designed for kinetically resolved fluorescence imaging of leaves and small plants both in a field and laboratory. The typical size of aninvestigated object is smaller than 3 x 3 cm. The HandyCam is a portable system that can run on sealed lead acid batteries in a convenient bag carried over the shoulder. In all applications, the camera allowsimaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols. The FluorCam software package includes a Wizard with the most frequently used experimental protocols. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences

Handy FluorCam 701MF Handy Version

 

Closed FluorCam 800MF
A New System for Multispectral Kinetic Fluorescence Imaging

Various light sources:
Super Bright LED panels with different wavelengths; for example, 390, 450, 470, 505, 570, 605, 630, 735 nm, and others.

  • STF - Single Turnover Flash.
  • IR 735 nm (FAR).
  • Ultra-high light intensity.
  • Actinic light up to 3,000 ƒÊmol(photons)/m2.s.
  • Super pulse up to 7,500 ƒÊmol(photons)/m2.s.
  • Variable excitation color.

 

Different emission bands:

  • 4 or 8 position filter wheel
  • Chlorophyll fluorescence, GFP, YFP, CY3, CY5, as well as other fluorescence colors..

 

Experiments and measured parameters:

  • QA reoxidation.
  • Quenching.
  • Kautsky induction and OJIP.
  • Fast fluorescence induction with 1 ƒÊs resolution.
  • Light curve.
  • Measured parameters (F0, FM, FV, F0f, FMf, FVf , QY(II)), Abs PAR-value.
  • Calculated parameters NPQ, FV/FM, FVf /FMf, Rfd, qN, qP, PAR-absorptivity, photosynthetic electron transport rate (PS), and others.

 

Sample size up to 13 cm x 13 cm:

  • 384-well plate, 96-well plate, Petri dish, etc.
  • Selectable shelf system for different plant sizes.
  • Dark room for adaptation.

 

Sample examples:

  • Leaves, plants, fruits, vegetables.
  • Cyanobacteria, green algae.
Closed FluorCam 800MF